Nucleic Acid Extraction Kit (A01)
Molao-motheo oa ho lemoha
Kamora ho lokolla DNA ea genomic ka ho arola lisele tse nang le lysis buffer, sefaha sa makenete se ka tlama ho DNA ea genomic sampoleng.Palo e nyane ea litšila tse monngoeng ke sefaha sa makenete e ka tlosoa ka sehlapo sa ho hlatsoa.Ho TE, sefaha sa makenete se ka lokolla DNA ea boundgenome, sa fumana DNA ea boleng bo holimo ea genome.Mokhoa ona o bonolo ebile o potlakile mme boleng ba DNA bo ntšitsoeng bo holimo, bo ka fihlelang tlhoko ea ho lemoha DNA methylation.Ho sa le joalo, lisebelisoa tsa ho hula tse thehiloeng holim'a sefaha sa makenete li ka tsamaellana le "automatic nucleic acid extract", ho kopana le mesebetsi e phahameng ea ho ntša nucleic acid.
Likarolo tse ka sehloohong tsa reagent
Likaroloana li bontšitsoe tafoleng ea 1:
Letlapa la 1 Likarolo tsa Reagent le Loading
| Lebitso la karolo | Likarolo tse ka sehloohong | Boholo (48) | Boholo (200) |
| 1. Tharollo ea Lysis | Guanidine hydrochloride, Tris | 11 mL / botlolo | 44 ml / botlolo |
| 2. Litharollo tsa ho hloekisa A | NaCl, Tris | 11 mL / botlolo | 44 ml / botlolo |
| 3. Litharollo tsa ho hloekisa B | NaCl, Tris | 13 mL / botlolo | 26.5mL/botlolo *2 |
| 4. Eluent | Tris, EDTA | 12 mL / botlolo | 44 ml / botlolo |
| 5. Tharollo ea Protease K | Protease K | 1.1mL / sengoathoana | 4.4mL / sengoathoana |
| 6. Ho emisoa ha lifaha tsa Magnetic 1 | Lifaha tsa makenete | 1.1mL / sengoathoana | 4.4mL / sengoathoana |
| 7. Litaelo tsa ho ntša li-reagents tsa nucleic acid |
| 1 khopi | 1 khopi |
Likarolo tse hlokoang ho ntšoa ha nucleic acid, empa ha li kenyellelitsoe ka har'a kit:
1. Reagent: Anhydrous ethanol, isopropanol le PBS;
2. Lisebelisoa: 50ml centrifuge tube le 1.5ml EP tube;
3. Thepa: Kettle ea ho hlapa metsi, pipettor, shelf ea makenete, centrifuge, poleiti e tebileng ea 96-hole (automatic), lisebelisoa tsa ho ntša li-nucleic acid (automatic).
Lintlha tsa motheo
Litlhoko tsa mohlala
1. Tšilafalo ea sefapano pakeng tsa lisampole e lokela ho qojoa pokellong ea mohlala le polokelo.
2.Ho fumanoa ho tla phethoa tlas'a polokelo ea matsatsi a 7 ea mocheso o potolohileng ka mor'a ho bokelloa ha cervical exfoliated cell sample (e seng e tsitsitseng).Ho sibolloa ho tla phethoa tlas'a polokelo ea matsatsi a 30 ea mocheso oa tikoloho ka mor'a ho bokella mohlala oa moroto;Ho sibolloa ho tla phethoa ka nako ka mor'a ho bokelloa ha lisampole tsa lisele tsa setso.
Tlhaloso ea ho paka:Li-pcs tse 200 / lebokose, li-pcs tse 48 / lebokose.
Maemo a polokelo:2-30 ℃
Nako ea ho sebetsa:Likhoeli tse 12
Sesebelisoa se sebetsang:Tianlong NP968-C sesebelisoa sa ho ntša nucleic acid, Tiangen TGuide S96 sesebelisoa sa ho ntša nucleic acid, GENE DIAN EB-1000 sesebelisoa sa ho ntša nucleic acid.
Setifikeiti sa rekoto ea sesebelisoa sa bongaka No./litlhoko tsa tekheniki ea sehlahisoa No.: HJXB No. 20210099.
Letsatsi la tumello le ho fetoloa ha litaelo:
Letsatsi la tumello: Nov. 18, 2021
