TAGMe DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Urothelial
LIEKETSENG LIHLAHISO
Nepahalo
E netefalitsoe ka lisampole tsa kliniki tse fetang 3500 lithutong tsa litsi tse ngata tse foufetseng habeli, sehlahisoa se na le 92.7% e ikhethang le kutloisiso ea 82.1%.
E loketseng
Theknoloji ea pele ea ho lemoha methylation ea Me-qPCR e ka phethoa ka mohato o le mong nakong ea lihora tse 3 ntle le phetoho ea bisulfite.
E sa hlaseleng
Ke 30 ml feela ea sampole ea moroto e hlokahalang ho lemoha mefuta e 3 ea mofetše, ho kenyelletsa le mofetše oa renal pelvis, mofets'e oa popelo, mofetše oa senya ka nako e le 'ngoe.
Maemo a kopo
Tlhahlobo e Thusang
Palo ea batho ba tšoeroeng ke hematuria e sa utloeng bohloko/ ba belaelloang hore ba na le mofetše (mofets'e oa mofets'e / mofets'e oa renal pelvis)
Tlhahlobo ea Kotsi ea Kankere
Opereishene/ chemotherapy-e hlokang baahi ba nang le urothelial carcinoma;
Tlhokomelo ea ho pheta-pheta
Palo ea batho ka mor'a ho sebetsa ka urothelial carcinoma
TŠEBELETSO TŠEBELETSO
Setsi sena se sebelisetsoa ho lemoha boleng ba in vitro ba hypermethylation ea lefutso la Urothelial Carcinoma (UC) mefuteng ea urothelial.Sephetho se setle se bontša kotsi e eketsehileng ea UC, e hlokang cystoscope le / kapa tlhahlobo ea histopathological.Ho fapana le hoo, liphetho tse mpe tsa liteko li bonts'a hore kotsi ea UC e tlase, empa kotsi e ke ke ea qheleloa ka thoko ka botlalo.Tlhahlobo ea ho qetela e lokela ho thehoa ho cystoscope le / kapa liphello tsa histopathological.
LEMOHELO MOLAOOA
Sesebelisoa sena se na le reagent ea ho ntša nucleic acid le reagent ea PCR.Asiti ea Nucleic e ntšoa ka mokhoa oa magnetic-bead-based.Setsi sena se ipapisitse le molao-motheo oa mokhoa oa PCR oa fluorescence quantitative, ho sebelisa methylation-specific real-time PCR reaction ho sekaseka template ea DNA, le ho bona ka nako e ts'oanang libaka tsa CpG tsa mofuta oa UC le letšoao la taolo ea boleng ka hare ho liphatsa tsa lefutso tsa G1 le G2.Tekanyo ea methylation ea UC gene, e bitsoang Me value, e baloa ho ea ka UC gene methylated DNA amplification Ct value le Ct value ea reference.UC gene hypermethylation boemo bo botle kapa bo bobe bo khethoa ho latela boleng ba Me.
DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Urothelial
| Kopo ea bongaka | Tlhahlobo e thusang ea kliniki ea lefu la urothelial;tlhahlobo ea katleho ea phekolo ea opereishene / chemotherapy;ho lekola ho khutla hape ka mor'a ho buuoa |
| Lefutso la ho lemoha | UC |
| Mofuta oa mohlala | Mohlala oa lisele tsa moroto (sediment ea moroto) |
| Mokhoa oa teko | Theknoloji ea Fluorescence quantitative PCR |
| Mehlala e sebetsang | ABI7500 |
| Tlhaloso ea ho paka | 48 liteko/kit |
| Maemo a polokelo | Kit A e lokela ho bolokoa ho 2-30 ℃ Kit B e lokela ho bolokoa ho -20±5℃ E sebetsa ho isa ho likhoeli tse 12. |
