TAGMe DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Mofets'e / Kankere ea Endometrial
LIEKETSENG LIHLAHISO
E sa hlaseleng
E sebetsa ka borashe ba popelo le disampole tsa Pap smear.
E loketseng
Theknoloji ea pele ea ho lemoha methylation ea Me-qPCR e ka phethoa ka mohato o le mong nakong ea lihora tse 3 ntle le phetoho ea bisulfite.
Esale pele
E fumaneha sethaleng sa precancerous.
Boiketsetso
E tsamaellana le software e hlophisitsoeng ea tlhahlobo ea sephetho, tlhaloso ea liphetho e itirile ebile e baloa ka kotloloho.
Maemo a kopo
Tlhahlobo ea pele
Batho ba phelang hantle
Tlhahlobo ea Kotsi ea Kankere
Palo ea batho ba kotsing e kholo (e nang le kotsi e kholo ea papillomavirus ea motho (hrHPV) kapa e ntle bakeng sa cytology ea cervical exfoliation / e ntle bakeng sa papillomavirus ea motho e kotsing e kholo (hrHPV) kapa e ntle bakeng sa cytology ea cervical exfoliation)
Tlhokomelo ea ho pheta-pheta
Palo ea batho ba prognostic
TŠEBELETSO TŠEBELETSO
Sesebelisoa sena se sebelisetsoa ho lemoha boleng ba in vitro ba hypermethylation ea gene PCDHGB7 mefuteng ea cervical.Bakeng sa mofetše oa popelo, sephetho se setle se bonts'a kotsi e eketsehileng ea sehlopha sa 2 kapa boemo bo holimo / bo tsoetseng pele ba cervical intraepithelial neoplasia (CIN2+, ho kenyelletsa CIN2, CIN3, adenocarcinoma in situ, le mofets'e oa popelo), e hlokang tlhahlobo e eketsehileng ea colposcopy le / kapa histopathological .Ho fapana le hoo, liphetho tse mpe tsa liteko li bonts'a hore kotsi ea CIN2 + e tlase, empa kotsi e ke ke ea qheleloa ka thoko ka botlalo.Tlhahlobo ea ho qetela e lokela ho thehoa ho colposcopy le / kapa liphello tsa histopathological.Ho feta moo, bakeng sa mofetše oa endometrial, phello e ntle e bontša kotsi e eketsehileng ea liso le kankere ea endometrial, e hlokang tlhahlobo e eketsehileng ea histopathological ea endometrium.Ho fapana le hoo, liphetho tse mpe tsa liteko li bontša hore kotsi ea ho ba le liso tsa endometrial precancerous le mofets'e e tlase, empa kotsi e ke ke ea qheleloa ka thoko ka botlalo.Tlhahlobo ea ho qetela e lokela ho thehoa ho latela liphello tsa tlhahlobo ea histopathological ea endometrium.
PCDHGB7 ke setho sa lelapa la protocadherin γ gene cluster.Protocadherin e fumanoe e laola lits'ebetso tsa likokoana-hloko tse kang ho ata ha lisele, potoloho ea lisele, apoptosis, tlhaselo, ho falla le autophagy ea lisele tsa tumor ka litsela tse sa tšoaneng tsa lipontšo, 'me ho khutsisa liphatsa tsa lefutso ho bakoang ke hypermethylation ea sebaka se khothalletsang ho amana haufi-ufi le ketsahalo le tsoelo-pele. mefuta e mengata ea kankere.Ho tlalehiloe hore hypermethylation ea PCDHGB7 e amahanngoa le mefuta e sa tšoaneng ea lihlahala, tse kang non-Hodgkin lymphoma, kankere ea matsoele, kankere ea molomo oa popelo, kankere ea endometrial le kankere ea senya.
LEMOHELO MOLAOOA
Sesebelisoa sena se na le reagent ea ho ntša nucleic acid le reagent ea PCR.Asiti ea Nucleic e ntšoa ka mokhoa oa magnetic-bead-based.Setsi sena se ipapisitse le molao-motheo oa mokhoa oa PCR oa fluorescence quantitative, ho sebelisa methylation-specific real-time PCR reaction ho sekaseka template ea DNA, 'me ka nako e le 'ngoe ho lemoha libaka tsa CpG tsa mofuta oa PCDHGB7 le letšoao la taolo ea boleng ka hare ho liphatsa tsa lefutso tsa G1 le G2.Boemo ba methylation ba PCDHGB7 ka sampuli, kapa boleng ba Me, bo baloa ho latela PCDHGB7 gene methylated DNA amplification Ct value le Ct value ea reference.PCDHGB7 gene hypermethylation boemo bo botle kapa bo bobe bo khethoa ho latela boleng ba Me.
