TAGMe DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Endometrial
LIEKETSENG LIHLAHISO
Nepahalo
E netefalitsoe ho feta lisampole tsa kliniki tse 800 lithutong tsa litsi tse ngata tse foufetseng habeli, sehlahisoa se na le 82.81% e ikhethang le kutloisiso ea 80.65%.
E loketseng
Theknoloji ea pele ea ho lemoha methylation ea Me-qPCR e ka phethoa ka mohato o le mong nakong ea lihora tse 3 ntle le phetoho ea bisulfite.
Esale pele
E fumaneha sethaleng sa precancerous.
Boiketsetso
E sebetsa ka borashe ba popelo le disampole tsa Pap smear.
TŠEBELETSO TŠEBELETSO
Sesebelisoa sena se sebelisetsoa ho lemoha boleng ba in vitro hypermethylation ea mofuta oa PCDHGB7 incervical specimens.Phello e ntle e bontša kotsi e eketsehileng ea ho ba le liso tsa endometrial precancerous le kankere, e hlokang tlhahlobo e eketsehileng ea histopathological ea endometrium.Ho fapana le hoo, liphetho tse mpe tsa liteko li bontša hore kotsi ea ho ba le liso tsa endometrial precancerous le mofets'e e tlase, empa kotsi e ke ke ea qheleloa ka thoko ka botlalo.Tlhahlobo ea ho qetela e lokela ho thehoa ho latela liphello tsa tlhahlobo ea histopathological ea endometrium.PCDHGB7 ke setho sa lelapa la protocadherin γ gene cluster.Protocadherin e fumanoe e laola lits'ebetso tsa likokoana-hloko tse kang ho ata ha lisele, potoloho ea lisele, apoptosis, tlhaselo, ho falla le autophagy ea lisele tsa tumor ka litsela tse sa tšoaneng tsa lipontšo, 'me ho khutsisa liphatsa tsa lefutso ho bakoang ke hypermethylation ea sebaka se khothalletsang ho amana haufi-ufi le ketsahalo le tsoelo-pele. mefuta e mengata ea kankere.Ho tlalehiloe hore hypermethylation ea PCDHGB7 e amahanngoa le mefuta e sa tšoaneng ea lihlahala, tse kang non-Hodgkin lymphoma, kankere ea matsoele, kankere ea molomo oa popelo, kankere ea endometrial le kankere ea senya.
LEMOHELO MOLAOOA
Sesebelisoa sena se na le reagent ea ho ntša nucleic acid le reagent ea PCR.Asiti ea Nucleic e ntšoa ka mokhoa oa magnetic-bead-based.Setsi sena se ipapisitse le molao-motheo oa mokhoa oa PCR oa fluorescence quantitative, ho sebelisa methylation-specific real-time PCR reaction ho sekaseka template ea DNA, 'me ka nako e le 'ngoe ho lemoha libaka tsa CpG tsa mofuta oa PCDHGB7 le letšoao la taolo ea boleng ka hare ho liphatsa tsa lefutso tsa G1 le G2.Boemo ba methylation ba PCDHGB7 ka sampuli, kapa boleng ba Me, bo baloa ho latela PCDHGB7 gene methylated DNA amplification Ct value le Ct value ea reference.PCDHGB7 gene hypermethylation boemo bo botle kapa bo bobe bo khethoa ho latela boleng ba Me.
Maemo a kopo
Tlhahlobo ea pele
Batho ba phelang hantle
Tlhahlobo ea Kotsi ea Kankere
Lihlopha tse kotsing e kholo (batho ba nang le mali a sa tloaelehang ka botšehaling ka mor'a ho khaotsa ho ilela khoeli, endometrial thickening, joalo-joalo)
Tlhokomelo ea ho pheta-pheta
Palo ea batho ba prognostic
Bohlokoa ba bongaka
Tlhahlobo ea pele bakeng sa batho ba phetseng hantle:Kankere ea endometrial le maqeba a precancerous a ka hlahlojoa ka nepo;
Tekanyetso ea kotsi bakeng sa baahi ba kotsing e kholo:Tlhahlobo ea kotsi e ka etsoa bakeng sa batho ba nang le mali a sa tloaelehang ka botšehaling le ho teteana ha endometrial ka mor'a ho khaotsa ho ilela khoeli ho thusa tlhahlobo ea bongaka;
Tekolo e lebelletsoeng ea ho ipheta ha batho:Ho ka etsoa tlhahlobo ea ho khutla ha batho ka mor'a ho buuoa ho thibela tieho ea phekolo e bakoang ke ho ipheta.
Pokello ea mohlala
Mokhoa oa ho etsa lisampole: Beha sampole ea popelo e lahloang karolong ea popelo, ka bonolo itlotsa borashe ba molomo oa pōpelo 'me u potolohe ka makhetlo a 4-5 ho ea ka tatellano ea oache, butle-butle u tlose borashe ba molomo oa popelo, u e behe ka har'a tharollo ea ho boloka lisele, 'me u e ngole bakeng sa tlhahlobo e latelang.
Paballo ea lisampole:Mehlala e ka bolokoa mocheso oa kamore ho fihlela matsatsi a 14, ho 2-8 ℃ ho fihlela likhoeli tse 2, le ho -20±5℃ ho fihlela likhoeli tse 24.
Mokhoa oa ho lemoha: Lihora tse 3 (Ntle le ts'ebetso ea matsoho)
TAGMe DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Endometrial
| Kopo ea bongaka | Tlhahlobo e thusang ea kliniki ea endometrial carcinoma |
| Lefutso la ho lemoha | PCDHGB7 |
| Mofuta oa mohlala | Mehlala ea popelo ea basali |
| Mokhoa oa teko | Theknoloji ea Fluorescence quantitative PCR |
| Mehlala e sebetsang | ABI7500 |
| Tlhaloso ea ho paka | 48 liteko/kit |
| Maemo a polokelo | Kit A e lokela ho bolokoa ho 2-30 ℃ Kit B e lokela ho bolokoa ho -20±5℃ E sebetsa ho isa ho likhoeli tse 12. |
Mabapi le rona
Epiprobe e na le mohaho o pharaletseng oa mehaho: Setsi sa tlhahiso ea GMP se akaretsa sebaka sa 2200 square metres, 'me se boloka tsamaiso ea boleng ba ISO13485, e finyellang litlhoko tsa tlhahiso ea mefuta eohle ea lihlahisoa tsa reagent tsa tlhahlobo ea liphatsa tsa lefutso;laboratori ea bongaka e akaretsa sebaka sa lisekoere-mithara tse 5400 'me e na le bokhoni ba ho etsa khoebo ea ho lemoha kankere ea methylation e le laboratori ea bongaka e tiisitsoeng ea mokha oa boraro.Ntle le moo, re na le lihlahisoa tse tharo tse fumanoeng setifikeiti sa CE, se koahelang mofetše oa popelo, mofets'e oa endometrial le tlhahlobo e amanang le mofetše oa urothelial.
Theknoloji ea ho lemoha limolek'hule tsa kankere ea Epiprobe e ka sebelisoa bakeng sa tlhahlobo ea kankere ea pele, tlhahlobo e thusang, tlhahlobo ea pele le ka morao ho ts'ebetso, tlhahlobo ea recrudescence, e tsamaisang mokhoa oohle oa ho hlahloba kankere le phekolo, ho fana ka tharollo e molemo ho lingaka le bakuli.
