TAGMe DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Urothelial
LIEKETSENG LIHLAHISO
Nepahalo
E netefalitsoe ka lisampole tsa kliniki tse fetang 3500 lithutong tsa litsi tse ngata tse foufetseng habeli, sehlahisoa se na le 92.7% e ikhethang le kutloisiso ea 82.1%.
E loketseng
Theknoloji ea pele ea ho lemoha methylation ea Me-qPCR e ka phethoa ka mohato o le mong nakong ea lihora tse 3 ntle le phetoho ea bisulfite.
E sa hlaseleng
Ke 30 ml feela ea sampole ea moroto e hlokahalang ho lemoha mefuta e 3 ea mofetše, ho kenyelletsa le mofetše oa renal pelvis, mofets'e oa popelo, mofetše oa senya ka nako e le 'ngoe.
Maemo a kopo
Tlhahlobo e thusang: Bakuli ba nang le mofetše oa urothelial ba ka hlahlojoa ka mokhoa o sa hlaseleng ho thusa tlhahlobo ea bongaka.
Tekolo ea katleho ea opereishene / chemotherapy: Lekola katleho ea opereishene / chemotherapy ho thusa ntlafatso ea bongaka ea phello ea kalafo.
Tlhokomelo ea ho ipheta ha batho ka mor'a ho buuoa:Bakuli ba nang le mofetše oa urothelial ba ka behoa leihlo bakeng sa ho khutla hape ka mokhoa o sa hlaseleng ka mokhoa o sa hlaseleng, e leng ho ntlafatsang ho mamela ha mokuli.
Pokello ea mohlala
Mokhoa oa ho etsa lisampole: Mokhoa oa ho etsa sampole: Bokella sampole ea moroto (moroto oa hoseng kapa moroto o sa reroang), eketsa motsoako o bolokang moroto 'me u kopanye hantle, o o boloke mochesong oa kamore ebe o o ngola tlhahlobo e latelang.
Paballo ea lisampole: Mehlala e ka bolokoa mocheso oa kamore ho fihlela matsatsi a 14, ho 2-8 ℃ ho fihlela likhoeli tse 2, le ho -20±5℃ ho fihlela likhoeli tse 24.
Mokhoa oa ho lemoha: Lihora tse 3 (Ntle le ts'ebetso ea matsoho)
DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Urothelial
| Kopo ea bongaka | Tlhahlobo e thusang ea kliniki ea lefu la urothelial;tlhahlobo ea katleho ea phekolo ea opereishene / chemotherapy;ho lekola ho khutla hape ka mor'a ho buuoa |
| Lefutso la ho lemoha | UC |
| Mofuta oa mohlala | Mohlala oa lisele tsa moroto (sediment ea moroto) |
| Mokhoa oa teko | Theknoloji ea Fluorescence quantitative PCR |
| Mehlala e sebetsang | ABI7500 |
| Tlhaloso ea ho paka | 48 liteko/kit |
| Maemo a polokelo | Kit A e lokela ho bolokoa ho 2-30 ℃Kit B e lokela ho bolokoa ho -20±5℃ E sebetsa ho isa ho likhoeli tse 12. |
