TAGMe DNA Methylation Detection Kits(qPCR) bakeng sa Kankere ea Popelo
LIEKETSENG LIHLAHISO
Nepahalo
E netefalitsoe ho feta 36000 lisampole tsa bongaka lithutong tsa litsi tse ngata tse sa boneng habeli, sehlahisoa se na le 94.3% e ikhethang le kutloisiso ea 96.0%.
E loketseng
Theknoloji ea pele ea ho lemoha methylation ea Me-qPCR e ka phethoa ka mohato o le mong nakong ea lihora tse 3 ntle le phetoho ea bisulfite.
Esale pele
Tlhahlobo ea mofetše oa molomo oa popelo e ka fetisetsoa sethaleng sa liso tsa boemo bo holimo (li-precancerous lesions).
Boiketsetso
E tsamaellana le software e hlophisitsoeng ea tlhahlobo ea sephetho, tlhaloso ea liphetho e itirile ebile e baloa ka kotloloho.
TŠEBELETSO TŠEBELETSO
Sesebelisoa sena se sebelisetsoa ho lemoha boleng ba in vitro ba hypermethylation ea gene PCDHGB7 mefuteng ea cervical.Sephetho se setle se bontša kotsi e eketsehileng ea sehlopha sa 2 kapa sa boemo bo holimo / se tsoetseng pele ho feta cervical intraepithelial neoplasia (CIN2 +, ho akarelletsa le CIN2, CIN3, adenocarcinoma in situ, le kankere ea molomo oa popelo), e hlokang colposcopy e eketsehileng le / kapa tlhahlobo ea histopathological.Ho fapana le hoo, liphetho tse mpe tsa liteko li bonts'a hore kotsi ea CIN2 + e tlase, empa kotsi e ke ke ea qheleloa ka thoko ka botlalo.Tlhahlobo ea ho qetela e lokela ho thehoa ho colposcopy le / kapa liphello tsa histopathological.PCDHGB7 ke setho sa lelapa la protocadherin γ gene cluster.Protocadherin e fumanoe e laola lits'ebetso tsa likokoana-hloko tse kang ho ata ha lisele, potoloho ea lisele, apoptosis, tlhaselo, ho falla le autophagy ea lisele tsa tumor ka litsela tse sa tšoaneng tsa lipontšo, 'me ho khutsisa liphatsa tsa lefutso ho bakoang ke hypermethylation ea sebaka se khothalletsang ho amana haufi-ufi le ketsahalo le tsoelo-pele. mefuta e mengata ea kankere.Ho tlalehiloe hore hypermethylation ea PCDHGB7 e amahanngoa le mefuta e sa tšoaneng ea lihlahala, tse kang non-Hodgkin lymphoma, kankere ea matsoele, kankere ea molomo oa popelo, kankere ea endometrial le kankere ea senya.
LEMOHELO MOLAOOA
Sesebelisoa sena se na le reagent ea ho ntša nucleic acid le reagent ea PCR.Asiti ea Nucleic e ntšoa ka mokhoa oa magnetic-bead-based.Setsi sena se ipapisitse le molao-motheo oa mokhoa oa PCR oa fluorescence quantitative, ho sebelisa methylation-specific real-time PCR reaction ho sekaseka template ea DNA, 'me ka nako e le 'ngoe ho lemoha libaka tsa CpG tsa mofuta oa PCDHGB7 le letšoao la taolo ea boleng ka hare ho liphatsa tsa lefutso tsa G1 le G2.Boemo ba methylation ba PCDHGB7 ka sampuli, kapa boleng ba Me, bo baloa ho latela PCDHGB7 gene methylated DNA amplification Ct value le Ct value ea reference.PCDHGB7 gene hypermethylation boemo bo botle kapa bo bobe bo khethoa ho latela boleng ba Me.
Maemo a kopo
Tlhahlobo ea pele
Batho ba phelang hantle
Tlhahlobo ea Kotsi ea Kankere
Palo ea batho ba kotsing e kholo (e nang le kotsi e kholo ea papillomavirus ea motho (hrHPV) kapa e ntle bakeng sa cytology ea cervical exfoliation)
Tlhokomelo ea ho pheta-pheta
Palo ea batho ba postoperative (ba nang le nalane ea maqeba a boemo bo holimo kapa mofets'e oa popelo)
Bohlokoa ba bongaka
Tlhahlobo ea pele bakeng sa batho ba phetseng hantle:Kankere ea popelo le maqeba a precancerous a ka hlahlojoa ka nepo
Tekanyetso ea kotsi ho baahi ba kotsing e kholo:Karolelano ea likotsi e ka etsoa ho batho ba nang le HPV ho tataisa tlhahlobo e latelang ea liteko
Ho beha leihlo khafetsa palo ea batho ba kamora ho buuoa:Ho ka etsoa tlhahlobo ea ho khutla ha batho ka mor'a ho buuoa ho thibela tieho ea phekolo e bakoang ke ho ipheta
Pokello ea mohlala
Mokhoa oa ho etsa lisampole: Beha sampole ea popelo e lahloang karolong ea popelo, ka bonolo itlotsa borashe ba molomo oa pōpelo 'me u potolohe ka makhetlo a 4-5 ho ea ka tatellano ea oache, butle-butle u tlose borashe ba molomo oa popelo, u e behe ka har'a tharollo ea ho boloka lisele, 'me u e ngole bakeng sa tlhahlobo e latelang.
Paballo ea lisampole:Mehlala e ka bolokoa mocheso oa kamore ho fihlela matsatsi a 14, ho 2-8 ℃ ho fihlela likhoeli tse 2, le ho -20±5℃ ho fihlela likhoeli tse 24.
Mokhoa oa ho lemoha: Lihora tse 3 (Ntle le ts'ebetso ea matsoho)
DNA Methylation Detection Kits (qPCR) bakeng sa Kankere ea Urothelial
| Kopo ea bongaka | Tlhahlobo e thusang ea kliniki ea mofetše oa popelo |
| Lefutso la ho lemoha | PCDHGB7 |
| Mofuta oa mohlala | Mehlala ea popelo ea basali |
| Mokhoa oa teko | Theknoloji ea Fluorescence quantitative PCR |
| Mohlala o sebetsang | ABI7500 |
| Tlhaloso ea ho paka | 48 liteko/kit |
| Maemo a polokelo | Kit A e lokela ho bolokoa ho 2-30 ℃ Kit B e lokela ho bolokoa ho -20±5℃ E sebetsa ho isa ho likhoeli tse 12 |
