Gargle Nucleic Acid Extraction Reagents
LEMOHELO MOLAOOA
Sehlahisoa haholo-holo se na le motsoako oa gargle, motsoako oa superparamagnetic nanospheres o monyehang haholo, le li-reagents tse ikhethang tsa lysis.Lifaha tse ikhethang tse kentsoeng tsa makenete li na le kamano e ntle bakeng sa likarolo tsa 'mele (ho kenyeletsoa livaerase tse sa lefelloeng le lisele tse tšoaelitsoeng ke vaerase).Ha u kopana le tharollo ea lysis, li-non-ionic cell / nucleus-membrane-breaking surfactants le protease inhibitors ka tharollo li ka thibela mosebetsi oa DNA / RNA enzyme, le ho tsitsisa nucleic acid.Lintho tsohle tsa nucleic acid likarolong tsa 'mele tsa gargle li lokolloa ka katleho ka har'a tharollo ea lysis, ka potlako li fumana nucleic acid.Lisampole tsa gargle tse sebelisang kit ena ha li hloke ho hloekisoa ha nucleic acid, e ka sebetsang ka kotloloho ho lisebelisoa tsa ho lemoha ha nucleic acid.
Likarolo tse ka sehloohong tsa reagent
Likaroloana li bontšitsoe tafoleng ea 1.
Letlapa la 1 Likaroloana le Loading in Kit
| Lebitso la karolo | Likarolo tse ka sehloohong | Boholo (1) | Boholo (10) | Boholo (30) | Boholo (50) |
| 1. Gargle A | NaCl | 8mL / tube | 8mL / tube *10 li-tubes | 8mL / tube *30 li-tubes | 8mL / tube *50 li-tubes |
| 2. 'Mokelli oa Gargle | PP | 1 sengoathoana | 10 likhomphutha | 30 likhomphutha | 50 likhomphutha |
| 3. Tharollo ea Matlafatso B | Lifaha tsa makenete | 2mL / tube | 2mL / tube *10 li-tubes | 2mL / tube *30 li-tubes | 2mL / tube *50 li-tubes |
| 4 Lysis Buffer C | Protease K | 0.2mL / sengoathoana | 0.2mL / sengoathoana * 10 likhomphutha | 0.2mL / sengoathoana * 30 likhomphutha | 0.2mL / sengoathoana * 50 likhomphutha |
| 5. Magnetic Cap | Magnet | 1 sengoathoana | 10 likhomphutha | 30 likhomphutha | 50 likhomphutha |
Likaroloana li sebetsa bakeng sa likhoeli tse 12.
Likarolo tse hlokoang ho ntšoa ha nucleic acid, empa ha li kenyellelitsoe ka har'a kit:
1. Lisebelisoa: 1.5ml EP tube;
2. Thepa: Bate ea metsi (kapa bate ea tšepe), li-pipette le centrifuge.
Lintlha tsa motheo
Litlhoko tsa mohlala:
1. E sebetsa ho ts'ebetso ea nucleic acid le ho ntlafatsa lisampole tsa gargle.
2. Mohlala oa gargle o tla ekeletsoa tharollong ea ho ruisa B ka nako ka mor'a ho bokella.Lifaha tsa makenete tse nkiloeng li tla fetisetsoa ho lysis buffer C hang-hang.Mehlala e kentsoeng ho lysis buffer C e ka bolokoa mocheso oa kamore.
Tlhaloso ea ho paka: 1 sengoathoana / lebokose, likhomphutha tse 10 / lebokose, likhomphutha tse 30 / lebokose, le likhomphutha tse 50 / lebokose.
Maemo a polokelo: Tharollo ea ho ntlafatsa B le tharollo ea lysis C e lokela ho bolokoa likhoeli tse 12 ho 2-8 ℃, 'me likarolo tse ling li ka bolokoa likhoeli tse 12 tlas'a mocheso oa kamore ea r;Thepa e ka tsamaisoa le ho bolokoa ka nakoana tlas'a mocheso oa tikoloho, o sa lokelang ho feta matsatsi a 5.
Nako ea ho sebetsa: Likhoeli tse 12
Setifikeiti sa rekoto ea sesebelisoa sa bongaka No./litlhoko tsa tekheniki ea sehlahisoa No.:HJXB No. 20220086.
Letsatsi la tumello le ntlafatso ea Litaelo:
Letsatsi la tumello: Oct. 26, 2022
