Nucleic Acid Extraction Kit (A02)
Molao-motheo oa ho lemoha
Kamora ho lokolla DNA ea genomic ka ho arola lisele tse nang le lysis buffer, sefaha sa makenete se ka tlama ho DNA ea genomic sampoleng.Palo e nyane ea litšila tse monngoeng ke sefaha sa makenete e ka tlosoa ka sehlapo sa ho hlatsoa.Ho TE, sefaha sa makenete se ka lokolla DNA ea boundgenome, sa fumana DNA ea boleng bo holimo ea genome.Mokhoa ona o bonolo ebile o potlakile mme boleng ba DNA bo ntšitsoeng bo holimo, bo ka fihlelang tlhoko ea ho lemoha DNA methylation.Ho sa le joalo, lisebelisoa tsa ho hula tse thehiloeng holim'a sefaha sa makenete li ka tsamaellana le "automatic nucleic acid extract", ho kopana le mesebetsi e phahameng ea ho ntša nucleic acid.
Likarolo tse ka sehloohong tsa reagent
Likaroloana li bontšitsoe tafoleng ea 1:
Letlapa la 1 Likarolo tsa Reagent le Loading
| Lebitso la karolo | Likarolo tse ka sehloohong | Boholo (48) | Boholo (200) |
| 1. Tšilo ea lijo A | Tris, SDS | 15.8 mL / botlolo | 66mL / botlolo |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL / botlolo | 66mL / botlolo |
| 3. Hlatsoa Buffer A | NaCl, Tris | 11 mL / botlolo | 44 ml / botlolo |
| 4. Hlatsoa Buffer B | NaCl, Tris | 13 mL / botlolo | 26.5mL/botlolo *2 |
| 5. TE | Tris, EDTA | 12 mL / botlolo | 44 ml / botlolo |
| 6. Tharollo ea Protease K | Protease K | 1.1mL / sengoathoana | 4.4mL / sengoathoana |
| 7. Ho emisoa ha lifaha tsa Magnetic 2 | Lifaha tsa makenete | 0.5mL / sengoathoana | 2.2mL / sengoathoana |
| 8. Litaelo tsa ho ntša li-reagents tsa nucleic acid | / | 1 khopi | 1 khopi |
Likarolo tse hlokoang ho ntšoa ha nucleic acid, empa ha li kenyellelitsoe ka har'a kit:
1. Reagent: Anhydrous ethanol, isopropanol, le PBS;
2. Lisebelisoa: 50mL centrifuge tube le 1.5mL EP tube;
3. Thepa: Bate ea metsi, li-pipette, shelf ea makenete, centrifuge, poleiti ea seliba sa 96 (automatic), lisebelisoa tsa ho ntša li-nucleic acid (automatic).
Lintlha tsa motheo
Litlhoko tsa mohlala:
1.Ho fumanoa ho tla phethoa tlas'a polokelo ea matsatsi a 7 ea mocheso o potolohileng ka mor'a ho bokelloa ha cervical exfoliated cell sample (e sa tsitsitseng).
2.Ho fumanoa ho tla phethoa tlas'a polokelo ea matsatsi a 30 ea mocheso o potolohileng ka mor'a ho bokelloa ha cervical exfoliated cell sample (e tsitsitseng).
3. Ho sibolloa ho tla phethoa tlas'a polokelo ea matsatsi a 30 ea mocheso oa tikoloho ka mor'a ho bokella mohlala oa moroto;Ho sibolloa ho tla phethoa ka nako ka mor'a ho bokelloa ha lisampole tsa lisele tsa setso.
Tlhaloso ea ho paka:Li-pcs tse 200 / lebokose, li-pcs tse 48 / lebokose.
Maemo a polokelo:2-30 ℃
Nako ea ho sebetsa:Likhoeli tse 12
Sesebelisoa se sebetsang:Tianlong NP968-C sesebelisoa sa ho ntša nucleic acid, Tiangen TGuide S96 sesebelisoa sa ho ntša nucleic acid, GENE DIAN EB-1000 sesebelisoa sa ho ntša nucleic acid.
Setifikeiti sa rekoto ea sesebelisoa sa bongaka No./litlhoko tsa tekheniki ea sehlahisoa No.:HJXB No. 20210100.
Letsatsi la tumello le ntlafatso ea litaelo:Letsatsi la tumello: Nov. 18, 2021
Mabapi le rona
Joalo ka khoebo ea theknoloji e phahameng e thehiloeng ka selemo sa 2018 ke litsebi tse phahameng tsa epigenetic, Epiprobe e shebane le tlhahlobo ea limolek'hule tsa mofets'e oa DNA methylation le indasteri e nepahetseng ea theranostic.Ka motheo o tebileng oa theknoloji, re ikemiselitse ho etella pele mehla ea lihlahisoa tse ncha ho felisa mofetše!
Ho ipapisitsoe le lipatlisiso tsa nako e telele tsa sehlopha sa Epiprobe, nts'etsopele le phetoho lefapheng la DNA methylation ka mahlale a morao-rao, a kopantsoeng le sepheo se ikhethileng sa DNA methylation ea mofets'e, re sebelisa algorithm e ikhethang e kopanyang data e kholo le mahlale a mahlale a maiketsetso. ka boikemelo ba nts'etsapele thekenoloji ea biopsy e sirelelitsoeng ka patent e ikhethileng.Ka ho hlahloba boemo ba methylation ea libaka tse khethehileng tsa likhechana tsa DNA tse sa lefelloeng sampoleng, mefokolo ea mekhoa ea khale ea tlhahlobo le mefokolo ea opereishene le lisampole tsa ho phunya lia qojoa, tse sa fihlelleng ho fumanoa ka nepo ha mofets'e oa pele, empa hape li thusa ho lekola nako ea nnete. ho hlaha ha mofets'e le matla a nts'etsopele.
